Institute of Clinical Pharmacology, University of Leipzig, Leipzig, Germany.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jan 1;878(1):107-11. doi: 10.1016/j.jchromb.2009.11.032.
As part of a joint clinical research project to study the effects of nicotine on the brain, a HPLC electrospray ionisation mass spectrometry method with a solid-phase extraction sample preparation was developed for the quantitative determination of nicotine and cotinine in human serum in volunteers. The measured concentrations of nicotine and cotinine were used as control for smoking behaviour. A X-Bridge-column from Waters, and a SSQ 7000 single quadropole mass spectrometer with a TSP liquid chromatographic system were used. The method includes a simple and robust sample preparation and this assay has been shown to be of a sufficient sensitivity for this application. The limits of quantification were 5 and 2ng/ml for cotinine and nicotine, respectively. A simultaneous study was conducted to measure nicotine receptor availability and the vigilance in the same group of volunteers.
作为一项联合临床研究项目的一部分，该研究旨在探讨尼古丁对大脑的影响。研究人员开发了一种高效液相色谱-电喷雾电离质谱法，结合固相萃取样品制备，用于定量测定志愿者血清中的尼古丁和可替宁。所测量的尼古丁和可替宁浓度可作为吸烟行为的对照。该方法使用了 Waters 的 X-Bridge 柱和 SSQ 7000 单四极杆质谱仪以及 TSP 液相色谱系统。该方法包括简单而稳健的样品制备，并且该测定方法的灵敏度足以满足该应用的需求。可替宁和尼古丁的定量下限分别为 5 和 2ng/ml。同时，还对同一组志愿者进行了尼古丁受体可及性和警觉性的同步研究。
