Department of Radiation Oncology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea.
J Radiat Res. 2009 Nov;50(6):545-52. doi: 10.1269/jrr.09066.
Irradiation causes the impaired proliferation of cells lining mucosal membranes. Epidermal growth factor (EGF) facilitates proliferation of various skin cells; however, the wound healing effects of EGF on radiation-damaged cells is less well known. To evaluate the effects of recombinant human EGF (rhEGF) on the proliferation of cells following irradiation, we tested two types of fibroblast cell lines and one keratinocyte cell line. The viable cell numbers were significantly increased by rhEGF treatment for 24 h immediately after 8 Gy of irradiation. The most effective dose of rhEGF was 10 nM in all cell lines used in this study. The percentage of BrdU-labeled cells was also significantly increased by rhEGF treatment. To evaluate the effects of rhEGF on radiation-induced oral mucosal damage in BALB/c mice, we systematically injected 1 mg/kg/day EGF for three days after 17 Gy of irradiation. Administered rhEGF ameliorated radiation-induced mucosal damage in vivo. rhEGF significantly increased the epithelial cell layer thickness and the proliferation of basal layer cells as detected by Ki-67 staining. Our results suggest that rhEGF can be a therapeutic treatment for radiation-induced wounds by stimulating the proliferation of fibroblasts and keratinocytes following irradiation.
辐射会导致黏膜细胞的增殖受损。表皮生长因子（EGF）促进各种皮肤细胞的增殖；然而，EGF 对辐射损伤细胞的愈合作用知之甚少。为了评估重组人 EGF（rhEGF）对辐射后细胞增殖的影响，我们测试了两种成纤维细胞系和一种角化细胞系。在 8Gy 辐射后立即用 rhEGF 处理 24 小时，可显著增加活细胞数量。在本研究中使用的所有细胞系中，rhEGF 的最有效剂量为 10nM。BrdU 标记细胞的百分比也因 rhEGF 处理而显著增加。为了评估 rhEGF 对 BALB/c 小鼠放射性口腔黏膜损伤的影响，我们在 17Gy 照射后连续 3 天每天系统注射 1mg/kg EGF。给予 rhEGF 可改善体内辐射诱导的黏膜损伤。rhEGF 通过 Ki-67 染色可显著增加上皮细胞层厚度和基底层细胞的增殖。我们的研究结果表明，rhEGF 可以通过刺激辐射后成纤维细胞和角化细胞的增殖来治疗辐射诱导的伤口。
