Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan.
EMBO J. 2010 Feb 3;29(3):586-96. doi: 10.1038/emboj.2009.362. Epub 2009 Dec 3.
The synaptonemal complex (SC) is a tripartite protein structure consisting of two parallel axial elements (AEs) and a central region. During meiosis, the SC connects paired homologous chromosomes, promoting interhomologue (IH) recombination. Here, we report that, like the CE component Zip1, Saccharomyces cerevisiae axial-element structural protein, Red1, can bind small ubiquitin-like modifier (SUMO) polymeric chains. The Red1-SUMO chain interaction is dispensable for the initiation of meiotic DNA recombination, but it is essential for Tel1- and Mec1-dependent Hop1 phosphorylation, which ensures IH recombination by preventing the inter-sister chromatid DNA repair pathway. Our results also indicate that Red1 and Zip1 may directly sandwich the SUMO chains to mediate SC assembly. We suggest that Red1 and SUMO chains function together to couple homologous recombination and Mec1-Tel1 kinase activation with chromosome synapsis during yeast meiosis.
联会复合体（SC）是一种由两个平行的轴丝元件（AEs）和一个中央区域组成的三分体蛋白结构。在减数分裂过程中，SC 连接配对的同源染色体，促进同源重组。在这里，我们报告说，与 CE 成分 Zip1 一样，酿酒酵母轴丝结构蛋白 Red1 可以结合小泛素样修饰物（SUMO）多聚链。Red1-SUMO 链相互作用对于起始减数分裂 DNA 重组是可有可无的，但对于 Tel1 和 Mec1 依赖性 Hop1 磷酸化是必不可少的，这通过防止姐妹染色单体 DNA 修复途径来确保同源重组。我们的结果还表明，Red1 和 Zip1 可能直接夹住 SUMO 链以介导 SC 组装。我们认为 Red1 和 SUMO 链一起发挥作用，将同源重组和 Mec1-Tel1 激酶激活与酵母减数分裂中的染色体联会联系起来。
