Stebbing Martin, Potocnik Simon, Ye Pinglu, Badoer Emilio
School of Medical Sciences, RMIT University, Melbourne, VIC, Australia.
Methods Mol Biol. 2010;611:173-82. doi: 10.1007/978-1-60327-345-9_14.
Confocal microscopy provides a powerful and efficient tool for studying the morphology of cells. Here we describe its use to study the morphology of neurons in the dorsal horn of the spinal cord either following electrophysiological studies in live tissue slices or in neurons filled with dye in fixed tissue sections following identification using retrograde tracing. The methods are broadly applicable to other cell types and can be combined with multiple label immunohistochemistry to study cellular constituents or with subsequent DAB staining to produce a permanent mount.
共聚焦显微镜为研究细胞形态提供了一种强大而高效的工具。在此，我们描述了其在以下两种情况下的应用：一是在活组织切片进行电生理研究后，用于研究脊髓背角神经元的形态；二是在使用逆行追踪法鉴定后，用于研究固定组织切片中充满染料的神经元的形态。这些方法广泛适用于其他细胞类型，并且可以与多重标记免疫组织化学相结合以研究细胞成分，或者与随后的DAB染色相结合以制作永久装片。
