Cancer Research Institute, Xiangya Medical School, Central South University, 110 Xiangya Road, Changsha, Hunan Province, China.
Pathol Oncol Res. 2010 Sep;16(3):319-26. doi: 10.1007/s12253-009-9226-1. Epub 2009 Dec 4.
The early diagnosis of colorectal cancer (CRC) is important because it is one of the most readily curable of all cancers, if detected early. However, the sensitivity of current markers is low. Immunostaining intensity for the monoclonal antibody Hb3 in CRC cell lines and tissues was stronger than in controls. Interestingly, this was associated with a low level of tumor differentiation. We used Hb3-coupled affinity chromatography to search for a corresponding Hb3 antigen as a candidate biomarker for early detection, and identified a Rho GTPase activating protein 6 (RhoGAP6) isoform 1 variant as an Hb3 antigen by mass spectrometry. Using reverse transcription polymerase chain reaction and western blot analysis, we confirmed that the expression levels of this variant were elevated in aberrant cells and tissues. Thus, the RhoGAP6 isoform 1 variant might serve as a biomarker for the development and progression of CRC.
结直肠癌（CRC）的早期诊断很重要，因为如果早期发现，它是所有癌症中最容易治愈的癌症之一。然而，目前的标志物的灵敏度较低。在 CRC 细胞系和组织中，针对单克隆抗体 Hb3 的免疫染色强度强于对照。有趣的是，这与肿瘤分化程度低有关。我们使用 Hb3 偶联亲和层析来寻找相应的 Hb3 抗原作为早期检测的候选生物标志物，并通过质谱鉴定出一种 Rho GTPase 激活蛋白 6（RhoGAP6）异构体 1 变体作为 Hb3 抗原。通过逆转录聚合酶链反应和 Western blot 分析，我们证实这种变体在异常细胞和组织中的表达水平升高。因此，RhoGAP6 异构体 1 变体可能是 CRC 发展和进展的生物标志物。
