Culex nigripalpus（双翅目：蚊科）部分酯酶基因的分离与空间表达分析。

Isolation and spatial expression analysis of a partial esterase gene of Culex nigripalpus (Diptera: Culicidae).

机构信息

Florida Medical Entomology Laboratory, University of Florida, 200 9th St. SE, Vero Beach, FL 32962, USA.

出版信息

J Med Entomol. 2009 Nov;46(6):1370-5. doi: 10.1603/033.046.0616.

DOI:10.1603/033.046.0616

PMID:19960682

Abstract

Mosquitoes play an important role as vectors for a variety of pathogens. Insecticide use for control means results in selection of tolerant/resistant mosquitoes. An esterase cDNA was isolated from Culex nigripalpus Theobald (Diptera: Culicidae), and its spatial expression was characterized. GenBank tblastx and VectorBase searches with the translation product of the longest cDNA fragment (397 bp, CN Temsha est-1) of the Cx. nigripalpus esterase revealed similarity (95%) to the estalpha2 esterase gene product of Culex pipiens quinquefasciatus Say, suggesting it is a member of the esterase family. Transcripts encoding Cx. nigripalpus esterase were detected in midgut tissue, thoraces, and abdomens of female Cx. nigripalpus, findings that may support possible roles in feeding, digestion, or reproduction. Gene expression studies to determine the role of Cx. nigripalpus esterase in the mosquito are being conducted.

摘要

蚊子在传播各种病原体方面发挥着重要作用。为控制目的而使用杀虫剂会导致耐受/抗性蚊子的选择。从三带喙库蚊（库蚊科）中分离到一种酯酶 cDNA，并对其空间表达进行了特征描述。用最长 cDNA 片段（397bp，CN Temsha est-1）的翻译产物对 Cx. nigripalpus 酯酶进行 GenBank tblastx 和 VectorBase 搜索，发现与库蚊五斑按蚊 Say 的 estalpha2 酯酶基因产物具有相似性（95%），表明它是酯酶家族的一员。在雌性三带喙库蚊的中肠组织、胸部和腹部检测到编码 Cx. nigripalpus 酯酶的转录本，这可能支持其在摄食、消化或繁殖中发挥作用的可能性。正在进行基因表达研究，以确定 Cx. nigripalpus 酯酶在蚊子中的作用。