Smartt Chelsea T, Richards Stephanie L, Anderson Sheri L, Erickson Jennifer S
University of Florida, Institute of Food and Agricultural Sciences, Florida Medical Entomology Laboratory, Vero Beach, Florida 32962, USA.
Am J Trop Med Hyg. 2009 Aug;81(2):258-63.
Alterations in gene expression in the midgut of female Culex pipiens quinquefasciatus exposed to blood meals containing 6.8 logs plaque-forming units/mL of West Nile virus (WNV) were studied by fluorescent differential display. Twenty-six different cDNAs exhibited reproducible differences after feeding on infected blood. Of these, 21 cDNAs showed an increase in expression, and 5 showed a decrease in expression as a result of WNV presence in the blood meal. GenBank database searches showed that one clone with increased expression, CQ G12A2, shares 94% identity with a leucine-rich repeat-containing protein from Cx. p. quinquefasciatus and 32% identity to Toll-like receptors from Aedes aegypti. We present the first cDNA clone isolated from female Cx. p. quinquefasciatus midgut tissue whose expression changes on exposure to WNV. This cDNA represents a mosquito gene that is an excellent candidate for interacting with WNV in Cx. p. quinquefasciatus and may play a role in disease transmission.
通过荧光差异显示技术，研究了暴露于含有6.8对数空斑形成单位/毫升西尼罗河病毒（WNV）血餐的致倦库蚊雌性中肠基因表达的变化。在吸食感染血液后，26种不同的cDNA表现出可重复的差异。其中，21种cDNA的表达增加，5种cDNA的表达因血餐中存在WNV而减少。GenBank数据库搜索显示，一种表达增加的克隆CQ G12A2与致倦库蚊富含亮氨酸重复序列的蛋白有94%的同一性，与埃及伊蚊的Toll样受体有32%的同一性。我们展示了从致倦库蚊雌性中肠组织分离出的第一个cDNA克隆，其表达在暴露于WNV时发生变化。该cDNA代表一个蚊子基因，是致倦库蚊中与WNV相互作用的优秀候选基因，可能在疾病传播中发挥作用。
