Department of Parasitology, Institute of Biological Sciences, Federal University of Minas Gerais, 31270-901 Belo Horizonte, Minas Gerais, Brazil.
J Med Entomol. 2009 Nov;46(6):1514-8. doi: 10.1603/033.046.0640.
Wistar rats (Rattus norvegicus) infested with Dermatobia hominis (L. Jr., 1781) had their axillary lymph nodes removed and histopathologically processed. Follicular hyperplasia in the germinal center was noted from 2 d postinfestation (dpi), exhibiting a high number of centerblasts, mitotic and apoptotic cells, and a thin parafollicular area. The paracortex showed hyperplasia rich in dendritic cells, immunoblasts, and endothelial venules, with diapedesis seen from 4 dpi onward. Hyperplasia of the medullar sinus also was first observed at this point, as well as dilated lymphatic sinus, lymph, macrophages, neutrophils, mast cells, and eosinophils. Medullar strings were expanded and filled with immunoblasts, mitotic cells, and plasmocytes. Lymphadenitis was not observed. The expression of mast cells was similar for both myiasis-affected and control rats but increased significantly (mastocytosis) at 7 and 15 d postlarval emergence (dple). Eosinophilia was observed at 4, 10, 15, 20, and 28 dpi as well as at 2, 7, and 15 dple, particularly on the last three observations of dpi and the earliest dple. This experimental approach allowed progressive tissue reactions in the lymph nodes to be monitored during myiasis, particularly those involving mast cells and eosinophils. These reactions abated and complete repair was observed at 60 dple.
感染狄氏曼氏蚴（L. Jr.，1781）的威斯塔大鼠（Rattus norvegicus）被切除了腋窝淋巴结，并进行了组织病理学处理。从感染后第 2 天（2 dpi）开始，观察到生发中心的滤泡增生，表现为大量中心母细胞、有丝分裂和凋亡细胞，以及薄的副皮质区。副皮质区表现出丰富的树突状细胞、免疫母细胞和内皮静脉增生，从第 4 天开始出现渗出。髓窦的增生也在此时首次观察到，同时还有扩张的淋巴窦、淋巴、巨噬细胞、中性粒细胞、肥大细胞和嗜酸性粒细胞。髓索扩张并充满免疫母细胞、有丝分裂细胞和浆细胞。未观察到淋巴结炎。受蝇蛆病影响和对照大鼠的肥大细胞表达相似，但在幼虫孵化后第 7 天和第 15 天（7 dple 和 15 dple）显著增加（肥大细胞增多症）。在第 4、10、15、20 和 28 天 dpi 以及第 2、7 和 15 天 dple 观察到嗜酸性粒细胞增多症，尤其是在 dpi 的最后三次观察和最早的 dple 观察中。这种实验方法允许在蝇蛆病期间监测淋巴结中的组织反应，特别是涉及肥大细胞和嗜酸性粒细胞的反应。这些反应减轻，并在第 60 天 dple 时观察到完全修复。
