Department of Parasitology Institute of Biological Sciences, Federal University of Minas Gerais, 31270-901 Belo Horizonte, Minas Gerais, Brazil.
J Med Entomol. 2010 Jan;47(1):95-8. doi: 10.1603/033.047.0113.
Adult males of Mus musculus each infested with four first-instar (L1) larvae of Dermatobia hominis (Linneaus, Jr.) were used as donors of larvae to other mice (recipients). Larvae at four (L1), six (early L2), 12 (L2), or 20 (L3) days postinfestation (dpi), were implanted into the skin of each recipient. Only two of 38 mice (5.3%) were refractory to implants and three died after implantation. Developmental times (pre- plus postimplantation) of implanted larvae were of similar duration to those in larvae that completed their development in the original mice. The L3 that emerged from implanted hosts developed to pupae and fertile adult specimens, whose L1 descendants were used to maintain the D. hominis life cycle in our laboratory. The model described here has several potential applications, including studies of the host relationship with specific instars and the development of management and control measures to combat this Neotropical myiasis.
成年雄性小家鼠（Mus musculus）每只感染 4 条第一龄（L1）的人肤蝇幼虫（Dermatobia hominis（Linneaus，Jr.）），作为幼虫的供体移植给其他老鼠（受体）。在感染后 4（L1）、6（早期 L2）、12（L2）或 20（L3）天时，将幼虫植入每个受体的皮肤中。在 38 只老鼠中，只有 2 只（5.3%）对植入有抗性，并且有 3 只在植入后死亡。植入幼虫的发育时间（植入前加植入后）与在原老鼠中完成发育的幼虫相似。从植入宿主中出现的 L3 发育为蛹和有生育能力的成虫，其 L1 后代被用于维持我们实验室中的人肤蝇生命周期。这里描述的模型有几个潜在的应用，包括研究宿主与特定龄期的关系以及制定管理和控制措施来对抗这种新热带蝇蛆病。
