补肾抗衰片对实验性动脉粥样硬化家兔NF-κB信号通路及炎症因子的影响

[Effect of bushen kangshuai tablet on NF-kappaB signal pathway and inflammation factors in rabbits with experimental atherosclerosis].

作者信息

Zhang Jun-Ping, Xu Ying-Zhi, Li Ming

机构信息

The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin.

出版信息

Zhongguo Zhong Xi Yi Jie He Za Zhi. 2009 Sep;29(9):816-20.

PMID:19960980

Abstract

OBJECTIVE

To observe the intervening effect and acting mechanism of Bushen Kangshuai Tablet (BKT) on rabbits' atherosclerosis (AS).

METHODS

Thirty-six white Japanese rabbits were randomly divided into four groups: 6 in the normal control group, and each 10 in the model group, the BKT group and the simvastatin group. The AS model was established by high fatty diet feeding from the 1st to the 10th week, combined with immune injury at the 2nd week and femoral arterial balloon tearing at the 4th week. The medication of BKT and simvastatin was given during the whole 10-week course. Levels of triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), interleukin-1 (IL-1), monocyte chemotactic protein-1 (MCP-1), and tumor necrosis factor-alpha (TNF-alpha) were measured at the beginning and the 3rd, 6th and 10th weekend of the experiment. The nuclear factor-kappa B (NF-kappaB) expression in the aortic wall, ratio of plaque area to intima area (PA/IA), intima thickness (IT), aortic intima/media thickness ratio (IT/MT) and intima hyperplasia index (IHI) were measured at the terminal of the experiment. The correlation analysis was conducted between serum lipids, inflammation factors and IHI.

RESULTS

Compared with the normal control group, all indices of the blood lipids and Inflammation factors measured at various time points, and the PA/IA, IT/MT ratios as well as IHI in the model group were higher (P<0.01, P<0.05). Compared with the model group, the levels of IL-1, MCP-1, TNF-alpha, NF-kappaB as well as the ratios of PA/IA, IT/MT and IHI were lower in the two treated groups at all time points after treatment (P<0.01 or P<0.05). Pearson correlation analysis showed positive correlation between TC with IL-1, and TNF-alpha with IHI in the model group (P< 0.05); also between TC with IHI, IL-1 with IHI, and TNF-alpha with IHI and IL-1 in the BKT group (P<0.05), while no correlation between blood lipids with inflammation factors was observed.

CONCLUSION

BKT could suppress the inflammation reaction in rabbits to prevent AS formation, the action is not directly correlated with the blood lipid level.

摘要

目的

观察补肾康衰片（BKT）对家兔动脉粥样硬化（AS）的干预作用及作用机制。

方法

将36只日本大耳白兔随机分为四组：正常对照组6只，模型组、BKT组和辛伐他汀组各10只。第1至10周采用高脂饮食喂养建立AS模型，第2周联合免疫损伤，第4周行股动脉球囊拉伤。整个10周疗程均给予BKT和辛伐他汀药物。在实验开始时及第3、6和10周末测量甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）、白细胞介素-1（IL-1）、单核细胞趋化蛋白-1（MCP-1）和肿瘤坏死因子-α（TNF-α）水平。实验结束时测量主动脉壁中核因子-κB（NF-κB）表达、斑块面积与内膜面积比值（PA/IA）、内膜厚度（IT）、主动脉内膜/中膜厚度比值（IT/MT）和内膜增生指数（IHI）；对血脂、炎症因子与IHI进行相关性分析。

结果

与正常对照组相比，模型组各时间点测量的血脂和炎症因子各项指标以及PA/IA、IT/MT比值和IHI均升高（P<0.01，P<0.05）。与模型组相比，两个治疗组治疗后各时间点IL-1、MCP-1、TNF-α、NF-κB水平以及PA/IA、IT/MT比值和IHI均降低（P<0.01或P<0.05）。Pearson相关性分析显示，模型组中TC与IL-1、TNF-α与IHI呈正相关（P<0.05）；BKT组中TC与IHI、IL-1与IHI、TNF-α与IHI和IL-1也呈正相关（P<0.05），而血脂与炎症因子之间未观察到相关性。