[肥大细胞脱颗粒过程中组胺、类胰蛋白酶和β-己糖胺酶之间关系的研究。]
[Investigation on the relationship among histamine, tryptase and beta-hexosaminidase in the process of mast cell degranulation.].
作者信息
Guo Yong-Chao, Li Zhen-Xing, Lin Hong
机构信息
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2009 Dec;25(12):1073-5.
AIM
To investigate the relationship among histamine, tryptase and beta-hexosaminidase in the process of mast cell release and search for the suitable index to describe mast cell degranulation.
METHODS
Mast cell degranulation was performed in vitro, in which histamine, tryptase and beta-hexosaminidase are detected by spectrophotometry.
RESULTS
The concordance was found to exit among the three mediums, furthermore the study discloses that more similarity was found between tryptase and beta-hexosaminidase. Histamine was difficult and inconstant to be detected.
CONCLUSION
Tryptase and beta-hexosaminidase are more suitable and accurately to reflect mast cell degranulation in vitro than histamine. Tryptase is the excellent index to detect mast cell degranulation in vitro.
目的
研究肥大细胞释放过程中组胺、类胰蛋白酶和β-己糖胺酶之间的关系，并寻找描述肥大细胞脱颗粒的合适指标。
方法
体外进行肥大细胞脱颗粒实验，采用分光光度法检测组胺、类胰蛋白酶和β-己糖胺酶。
结果
发现这三种介质之间存在一致性，此外研究还表明类胰蛋白酶和β-己糖胺酶之间的相似性更高。组胺检测困难且不稳定。
结论
与组胺相比，类胰蛋白酶和β-己糖胺酶更适合且准确地反映体外肥大细胞脱颗粒情况。类胰蛋白酶是体外检测肥大细胞脱颗粒的优良指标。
Zotero 插件