Sohn Eun-Hwa, Jang Seon-A, Joo Haemi, Park Sulkyoung, Kang Se-Chan, Lee Chul-Hoon, Kim Sun-Young
Department of Pediatrics, College of Medicine, Hanyang University, Seoul, 133-792, Korea.
Clin Mol Allergy. 2011 Feb 8;9(1):4. doi: 10.1186/1476-7961-9-4.
Atopic dermatitis is a chronic, allergic inflammatory skin disease that is accompanied by markedly increased levels of inflammatory cells, including eosinophils, mast cells, and T cells. Arctium lappa L. is a traditional medicine in Asia. This study examined whether a butanol extract of A. lappa (ALBE) had previously unreported anti-allergic or anti-inflammatory effects.
This study examined the effect of ALBE on the release of β-hexosaminidase in antigen-stimulated-RBL-2H3 cells. We also evaluated the ConA-induced expression of IL-4, IL-5, mitogen-activated protein kinases (MAPKs), and nuclear factor (NF)-κB using RT-PCR, Western blotting, and ELISA in mouse splenocytes after ALBE treatment.
We observed significant inhibition of β-hexosaminidase release in RBL-2H3 cells and suppressed mRNA expression and protein secretion of IL-4 and IL-5 induced by ConA-treated primary murine splenocytes after ALBE treatment. Additionally, ALBE (100 μg/mL) suppressed not only the transcriptional activation of NF-κB, but also the phosphorylation of MAPKs in ConA-treated primary splenocytes.
These results suggest that ALBE inhibits the expression of IL-4 and IL-5 by downregulating MAPKs and NF-κB activation in ConA-treated splenocytes and supports the hypothesis that ALBE may have beneficial effects in the treatment of allergic diseases, including atopic dermatitis.
特应性皮炎是一种慢性过敏性炎症性皮肤病，伴有炎症细胞水平显著升高，包括嗜酸性粒细胞、肥大细胞和T细胞。牛蒡是亚洲的一种传统药物。本研究探讨了牛蒡丁醇提取物（ALBE）是否具有此前未报道的抗过敏或抗炎作用。
本研究检测了ALBE对抗原刺激的RBL-2H3细胞中β-己糖胺酶释放的影响。我们还在ALBE处理后的小鼠脾细胞中，使用RT-PCR、蛋白质印迹法和酶联免疫吸附测定法评估了刀豆蛋白A（ConA）诱导的白细胞介素-4（IL-4）、白细胞介素-5（IL-5）、丝裂原活化蛋白激酶（MAPK）和核因子-κB（NF-κB）的表达。
我们观察到ALBE处理后，RBL-2H3细胞中β-己糖胺酶的释放受到显著抑制，ConA处理的原代小鼠脾细胞诱导的IL-4和IL-5的mRNA表达及蛋白分泌也受到抑制。此外，ALBE（100μg/mL）不仅抑制了ConA处理的原代脾细胞中NF-κB的转录激活，还抑制了MAPK的磷酸化。
这些结果表明，ALBE通过下调ConA处理的脾细胞中MAPK和NF-κB的激活来抑制IL-4和IL-5的表达，并支持ALBE可能对包括特应性皮炎在内的过敏性疾病治疗具有有益作用的假说。
