Laboratorio de Inmuno-Biología Molecular, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
Hum Immunol. 2010 Feb;71(2):151-7. doi: 10.1016/j.humimm.2009.11.011. Epub 2009 Dec 16.
Little is known about innate immunity of neonates, particularly for very-preterm ones, which are more susceptible to immunologic damage due to their immature immune response. This cross-sectional, descriptive study in umbilical cord blood mononuclear cells describes the differences in innate immune response between 64 healthy neonates of different gestational ages (very-preterm, preterm, full-term). CD14(+) monocytes cultured with lipopolysaccharide (LPS) or LPS + interferon-gamma showed significant lower human leukocyte antigen-DR percentages for the very-preterm group in both unstimulated and LPS-stimulated cells. No differences were found for CD40(+)-cell percentages. We observed an increase in CD80 and a decrease in CD86 within all groups when stimulated with LPS or LPS + interferon-gamma. Interleukin-12 production was lower in very-preterm neonates. Adhesion capability of neonatal monocytes was similar and independent of gestational age. In summary, very-preterm-neonatal monocytes do not completely respond to LPS and, therefore, have diminished functions compared with preterm or full-term neonates.
关于新生儿的先天免疫，人们知之甚少，尤其是对于非常早产儿，由于其不成熟的免疫反应，他们更容易受到免疫损伤。这项在脐带血单核细胞中进行的横断面描述性研究，描述了不同胎龄（极早产儿、早产儿、足月儿）的 64 名健康新生儿先天免疫反应的差异。用脂多糖（LPS）或 LPS + 干扰素-γ培养的 CD14（+）单核细胞，在未刺激和 LPS 刺激的细胞中，极早产儿组的人白细胞抗原-DR 百分比显著降低。CD40（+）细胞百分比无差异。当用 LPS 或 LPS + 干扰素-γ刺激时，我们观察到所有组中的 CD80 增加和 CD86 减少。与早产儿或足月儿相比，极早产儿产生的白细胞介素 12 较少。新生儿单核细胞的黏附能力相似，且与胎龄无关。总之，极早产儿的单核细胞对 LPS 的反应不完全，因此与早产儿或足月儿相比，其功能减弱。
