Department of Obstetrics and Gynecology, University of Greifswald, Greifswald, Germany.
Fertil Steril. 2010 May 15;93(8):2581-7. doi: 10.1016/j.fertnstert.2009.10.025. Epub 2009 Dec 3.
To examine the impact of unfractionated heparin and low-molecular-weight heparins (LMWHs) on the decidualization of human endometrial stromal cells (ESCs) in vitro.
In vitro experiment.
Research laboratory at a medical university center.
PATIENT(S): Premenopausal women undergoing hysterectomy for benign reasons.
INTERVENTION(S): The ESCs were isolated from hysterectomy specimens, decidualized in vitro using progesterone and 17beta-estradiol, and incubated with unfractionated heparin and three different LMWHs.
MAIN OUTCOME MEASURE(S): Insulin-like growth factor-binding protein (IGFBP) 1, PRL, and insulin-like growth factor (IGF) I were measured using ELISA and real-time reverse-transcription polymerase chain reaction. Cell viability was determined by a fluorometric assay. Intracellular cyclic adenosine 3',5'-monophosphate (cAMP) was measured using a luminescent assay.
RESULT(S): Heparin dose- and time-dependently delayed the production of IGFBP-1 and amplified the levels of PRL and IGF-I in ESCs during decidualization in vitro. Similar effects were seen under the influence of the three different LMWHs. Intracellular cAMP was increased in decidualizing ESCs under the influence of heparin and LMWHs.
CONCLUSION(S): Unfractionated heparin as well as LMWHs are able to modulate the decidualization of human ESCs in vitro and therefore might be useful to control endometrial differentiation and receptivity in assisted reproduction.
研究未分级肝素和低分子肝素（LMWH）对体外人子宫内膜基质细胞（ESC）蜕膜化的影响。
体外实验。
医科大学中心的研究实验室。
因良性原因接受子宫切除术的绝经前妇女。
从子宫切除术标本中分离 ESC，用孕激素和 17β-雌二醇体外蜕膜化，并与未分级肝素和三种不同的 LMWH 孵育。
采用 ELISA 和实时逆转录聚合酶链反应测定胰岛素样生长因子结合蛋白（IGFBP）1、PRL 和胰岛素样生长因子（IGF）I。通过荧光测定法测定细胞活力。通过发光测定法测定细胞内环磷酸腺苷（cAMP）。
肝素剂量和时间依赖性地延迟 IGFBP-1 的产生，并在体外蜕膜化过程中放大 PRL 和 IGF-I 的水平。三种不同的 LMWH 也有类似的影响。肝素和 LMWH 影响下的蜕膜化 ESC 中细胞内 cAMP 增加。
未分级肝素和低分子肝素均可调节体外人 ESC 的蜕膜化，因此可能有助于控制辅助生殖中的子宫内膜分化和接受性。
