Nanoscience Center, Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, Finland.
Chem Phys Lipids. 2010 Feb;163(2):218-27. doi: 10.1016/j.chemphyslip.2009.11.007. Epub 2009 Dec 3.
The effects of tri- and monoglycerides on phospholipid (POPC) membranes were studied using spectroscopical methods. Triolein was found to form two types of POPC-rich membranes, both with POPC or as a three-component system with monopalmitin. These two membrane types were determined as co-existing phases based on their spontaneous and stable separation and named heavy and light phase according to their sedimentation behaviour. Marked differences were seen in the physical properties of these phases, even though only minor compositional variation was detected. The light, less polar phase was found to be less ordered and more fluid and seemed to allow significantly lower amount of water penetration into the membrane-water interface than pure POPC membrane. The heavy phase, apart from their slightly altered water penetration, resembled more a pure POPC membrane. As triglycerides are present in lysosomal membranes, the present results can be seen as an implication for polarity-based water permeability barrier possibly contributing to the integrity of lysosomes.
使用光谱学方法研究了三酰基和单酰基甘油对磷脂（POPC）膜的影响。发现三油酸甘油酯形成两种富含 POPC 的膜，一种是含有 POPC 的膜，另一种是含有单棕榈酸甘油酯的三组分体系。这两种膜类型基于它们的自发和稳定分离被确定为共存相，并根据它们的沉淀行为命名为重相和轻相。尽管仅检测到微小的组成变化，但这些相的物理性质存在明显差异。发现较不极性的轻相无序性更小、流动性更大，似乎允许进入膜-水界面的水量明显低于纯 POPC 膜。除了水渗透略有改变外，重相更类似于纯 POPC 膜。由于甘油三酯存在于溶酶体膜中，因此目前的结果可以被视为对极性基础的水渗透性屏障的暗示，该屏障可能有助于溶酶体的完整性。
