Bartucci Rosa, Erilov Denis A, Guzzi Rita, Sportelli Luigi, Dzuba Sergei A, Marsh Derek
Dipartimento di Fisica, Università della Calabria, Arcavacata di Rende (CS), Italy.
Chem Phys Lipids. 2006 Jun;141(1-2):142-57. doi: 10.1016/j.chemphyslip.2006.02.009. Epub 2006 Mar 13.
Recently, developments in time-resolved spin-label electron spin resonance (ESR) spectroscopy have contributed considerably to the study of biomembranes. Two different applications of electron spin echo spectroscopy of spin-labelled phospholipids are reviewed here: (1) the use of partially relaxed echo-detected ESR spectra to study the librational lipid-chain motions in the low-temperature phases of phospholipid bilayers; (2) the use of electron spin echo envelope modulation spectroscopy to determine the penetration of water into phospholipid membranes. Results are described for phosphatidylcholine bilayer membranes, with and without equimolar cholesterol, that are obtained with phosphatidylcholine spin probes site-specifically labelled throughout the sn-2 chain.
最近，时间分辨自旋标记电子自旋共振（ESR）光谱技术的发展极大地推动了生物膜的研究。本文综述了自旋标记磷脂的电子自旋回波光谱的两种不同应用：（1）利用部分弛豫的回波检测ESR光谱研究磷脂双层低温相中脂质链的振动运动；（2）利用电子自旋回波包络调制光谱测定水进入磷脂膜的渗透率。文中描述了使用在整个sn-2链上进行位点特异性标记的磷脂酰胆碱自旋探针，对含有和不含有等摩尔胆固醇的磷脂酰胆碱双层膜所获得的结果。
