Department of Botany and Zoology, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa.
Ecotoxicol Environ Saf. 2010 Mar;73(3):407-14. doi: 10.1016/j.ecoenv.2009.03.017. Epub 2009 Dec 4.
We investigated the effect of clay content on the bioavailability of copper to the earthworm Eisenia fetida, in the laboratory for 28 days using OECD artificial soil adjusted to 5%, 20% and 40% clay. Mortality, growth, cocoon production and internal copper concentrations were assessed in worms, and total, DTPA and CaCl(2) extractable copper in the substrates were also determined. The results showed that with increased clay content, there was significant reduction in internal Cu concentration of worms as well as toxicity of copper as shown by data for growth and mortality. However, internal copper concentrations of worms could not explain fully the observed toxicity in this study probably because of Cu regulation. Among the three extraction methods, DTPA extraction revealed the strongest relationship between partitioning and toxicity of Cu in this study. DTPA extract is thus a promising surrogate measure of bioavailability of Cu to earthworms. It is concluded that clay content has significant influence on the bioavailability of copper to earthworms and other similar species.
我们研究了在实验室条件下，用 OECD 人工土壤调整到 5%、20%和 40%的粘土含量，对赤子爱胜蚓（Eisenia fetida）体内铜的生物有效性的影响，为期 28 天。在蚯蚓体内评估了死亡率、生长、茧产量和体内铜浓度，同时还测定了基质中的总铜、DTPA 可提取铜和 CaCl2 可提取铜。结果表明，随着粘土含量的增加，蚯蚓体内铜的浓度以及铜的毒性（表现为生长和死亡率数据）显著降低。然而，蚯蚓体内铜的浓度并不能完全解释本研究中观察到的毒性，这可能是因为铜的调节。在三种提取方法中，DTPA 提取法揭示了本研究中铜的分配和毒性之间最强的关系。因此，DTPA 提取法是一种很有前途的铜对蚯蚓生物有效性的替代测量方法。研究结论认为，粘土含量对铜对蚯蚓和其他类似物种的生物有效性有显著影响。
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