Sivakumar Ramalingam, Babu Pon Velayutham Anandh, Shyamaladevi Chennam Srinivasulu
Department of Biochemistry, University of Madras, Guindy Campus, Chennai 600 025, India .
Exp Toxicol Pathol. 2011 Jan;63(1-2):137-42. doi: 10.1016/j.etp.2009.10.008. Epub 2009 Dec 4.
The protective effect of aspartate and glutamate in isoproterenol induced myocardial infarction (MI) was investigated in experimental animals. Male albino wistar rats were pretreated with aspartate [100mg (kg body weight)-1 day-1] or glutamate [100mg (kg body weight)-1 day-1] intraperitoneally for a period of 7 days. Following amino acid treatment, MI was induced in rats by subcutaneous injection of isoproterenol [200mg (kg body weight)-1 day-1] for 2 days. After 24h following the last injection, the animals were sacrificed and the biochemical analysis was carried out. The activities of cardiac marker enzymes (alanine transaminase, aspartate transaminase, lactate dehydrogenase and creatine phosphokinase) were increased significantly (P<0.05) in the serum of MI induced rats as compared to control rats. The levels of glutathione and mitochondrial ATP and the activities of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, glutathione transferase and glutathione reductase) were decreased whereas lipid peroxides increased significantly (P<0.05) in the heart of MI induced rats as compared to control rats. However, pretreatment with aspartate or glutamate to MI induced rats significantly (P<0.05) reduced the activities of cardiac marker enzymes and increased the activities of antioxidant enzymes as compared to MI induced rats. Aspartate or glutamate pretreatment also increased the levels of glutathione and mitochondrial ATP while decreased the level of lipid peroxides in the cardiac tissue. The overall effects of aspartate and glutamate in reducing the oxidative stress in MI induced rats are similar. There was no significant difference between the control rats and aspartate or glutamate treated control rats. The present study shows that aspartate and glutamate could reduce oxidative stress in MI induced rats.
在实验动物中研究了天冬氨酸和谷氨酸对异丙肾上腺素诱导的心肌梗死（MI）的保护作用。雄性白化Wistar大鼠腹腔注射天冬氨酸[100mg/（kg体重）-1天-1]或谷氨酸[100mg/（kg体重）-1天-1]预处理7天。氨基酸处理后，皮下注射异丙肾上腺素[200mg/（kg体重）-1天-1]2天诱导大鼠发生心肌梗死。最后一次注射后24小时，处死动物并进行生化分析。与对照大鼠相比，MI诱导大鼠血清中心脏标志物酶（丙氨酸转氨酶、天冬氨酸转氨酶、乳酸脱氢酶和肌酸磷酸激酶）的活性显著增加（P<0.05）。与对照大鼠相比，MI诱导大鼠心脏中谷胱甘肽、线粒体ATP水平以及抗氧化酶（超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽转移酶和谷胱甘肽还原酶）的活性降低，而脂质过氧化物显著增加（P<0.05）。然而，与MI诱导大鼠相比，用天冬氨酸或谷氨酸预处理MI诱导大鼠可显著（P<0.05）降低心脏标志物酶的活性并增加抗氧化酶的活性。天冬氨酸或谷氨酸预处理还增加了心脏组织中谷胱甘肽和线粒体ATP的水平，同时降低了脂质过氧化物的水平。天冬氨酸和谷氨酸在降低MI诱导大鼠氧化应激方面的总体效果相似。对照大鼠与天冬氨酸或谷氨酸处理的对照大鼠之间无显著差异。本研究表明，天冬氨酸和谷氨酸可降低MI诱导大鼠的氧化应激。
