Cumming R, Eccleston D, Steiner A
J Cyclic Nucleotide Res. 1977 Aug;3(4):275-82.
The technique of cyclic nucleotide fluorescence immunohistochemistry has been applied for the specific localization of cyclic GMP in rat cerebellum. We report immunofluorescence associated with fibres and membranes, contrasting with previously reported cytoplasmic localization of cyclic AMP in different cell populations, using a similar technique. We have been unable to detect changes in cyclic GMP staining in response to post-mortem changes, harmaline and pentobarbitone administration. A role of cyclic GMP is suggested in membrane ion transport.
环核苷酸荧光免疫组织化学技术已被应用于大鼠小脑中环鸟苷酸（cGMP）的特异性定位。我们报告了与纤维和膜相关的免疫荧光，这与之前使用类似技术报道的不同细胞群体中环磷酸腺苷（cAMP）的细胞质定位形成对比。我们未能检测到死后变化、给予槟榔碱和戊巴比妥后cGMP染色的变化。提示cGMP在膜离子转运中起作用。
