实时逆转录聚合酶链反应检测 2009 年甲型 H1N1 流感病毒。
Detection of pandemic influenza A/H1N1/2009 virus by real-time reverse transcription polymerase chain reaction.
机构信息
Beijing Institute of Genomics, Chinese Academy of Sciences, Airport Industry B6, Beijing 101300, China.
出版信息
J Virol Methods. 2010 May;165(2):294-6. doi: 10.1016/j.jviromet.2009.11.033. Epub 2009 Dec 4.
The first case of pandemic influenza A/H1N1/2009 infection was reported in Mexico in mid-April, 2009, and to date the new H1N1 virus has spread to over 160 countries. Therefore, it is important to obtain reliable epidemiological data on the spread of this virus by novel molecular methods can be developed in detection of this new infectious agent. A new method was developed for detection of the pandemic influenza A/H1N1/2009 using real-time reverse transcription polymerase chain reaction (RT-PCR). Direct comparison with the specific primers and probes recommended by the World Health Organization (WHO) demonstrates that the new method has a much greater sensitivity, and may thus be employed as a rapid, alternative method in detecting of the pandemic influenza A/H1N1/2009 virus.
首例 2009 年甲型 H1N1 流感疫情于 2009 年 4 月中旬在墨西哥报告，截至目前，新型 H1N1 病毒已传播至 160 多个国家。因此，采用新的分子方法获得有关这种病毒传播的可靠流行病学数据对于检测这种新型传染病至关重要。本研究建立了一种新的实时逆转录聚合酶链反应（RT-PCR）检测方法，用于检测大流行流感 A/H1N1/2009。与世界卫生组织（WHO）推荐的特异性引物和探针的直接比较表明，该方法具有更高的灵敏度，因此可作为一种快速、替代的方法用于检测大流行流感 A/H1N1/2009 病毒。
Zotero 插件