[通过实时聚合酶链反应鉴定甲型H1N1大流行性流感病毒]
[Identification of a pandemic influenza virus A/H1N1 by real-time PCR].
作者信息
Spyrydonov V H, Ishchenko L M, Martynenko D L, Mel'nychuk S D, Mel'nychuk M D
出版信息
Mikrobiol Z. 2010 Jan-Feb;72(1):47-51.
PMID:20364716
Abstract
The WHO approved protocol of pandemic influenza virus A/H1N1 identification by the method of one-step RT-PCR was adopted. The cost of research was decreased due to adoption of RNA purification method and utilizing of the common enzyme systems for RT-PCR.
摘要
采用了世界卫生组织批准的通过一步法逆转录聚合酶链反应（RT-PCR）鉴定甲型H1N1大流行性流感病毒的方案。由于采用了RNA纯化方法并利用了RT-PCR的通用酶系统，研究成本降低了。
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