State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
Bioresour Technol. 2010 Mar;101(6):2053-8. doi: 10.1016/j.biortech.2009.11.031. Epub 2009 Dec 5.
The optimization of culture condition for enhanced hydrogen production by Thermoanaerobacterium thermosaccharolyticum W16 was conducted using statistical experimental design and analysis. Plackett-Burman design was first used to screen the most important variables influencing hydrogen production, and subsequently central composite design was adopted to investigate the optimum value of the selected factors for achieving maximum hydrogen yield. Experimental results showed that xylose, phosphate buffer, and yeast extract had significant influence on hydrogen production and the maximum hydrogen yield of 2.39 mol/mol xylose was predicted when the concentrations of xylose, phosphate buffer, and yeast extract were 12.24 g/L, 0.170 M, and 4.11 g/L, respectively. The results were further verified by repeated experiments under optimal conditions. The excellent correlation between predicted and measured values further confirmed the validity and practicability of this statistical optimum strategy.
采用统计实验设计和分析方法对嗜热解糖梭菌 W16 产氢的最佳培养条件进行了优化。首先采用 Plackett-Burman 设计筛选出影响产氢的最重要变量，然后采用中心组合设计研究了为获得最大产氢量所选因素的最佳值。实验结果表明，木糖、磷酸盐缓冲液和酵母提取物对产氢有显著影响，当木糖、磷酸盐缓冲液和酵母提取物的浓度分别为 12.24 g/L、0.170 M 和 4.11 g/L 时，最大产氢量预测值为 2.39 mol/mol 木糖。在最佳条件下进行的重复实验进一步验证了该结果。预测值与实测值之间的良好相关性进一步证实了该统计最优策略的有效性和实用性。
