使用β粒子气体探测器对组织切片中氚标记化合物进行定位和定量：与胶片放射自显影法的比较
Localization and quantitation of tritiated compounds in tissue sections with a gaseous detector of beta particles: comparison with film autoradiography.
作者信息
Tribollet E, Dreifuss J J, Charpak G, Dominik W, Zaganidis N
机构信息
Department of Physiology, University Medical Centre, Geneva, Switzerland.
出版信息
Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1466-8. doi: 10.1073/pnas.88.4.1466.
Abstract
Quantitative analysis of tritium polymer standards and of brain sections labeled with tritiated vasopressin was carried out by using a gaseous detector of beta particles designed for this purpose. The gaseous detector showed major advantages compared with film autoradiography: the linearity and the large dynamic range of intensity measurements as well as the short time needed for data acquisition.
摘要
使用专门为此设计的β粒子气体探测器，对氚聚合物标准品以及用氚标记的血管加压素标记的脑切片进行了定量分析。与胶片放射自显影相比，该气体探测器具有主要优势：强度测量的线性和大动态范围，以及数据采集所需的短时间。
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