Nagata A, Miura M, Komoda T
Department of Biochemistry, Saitama Medical School, Moroyama, Japan.
Tumour Biol. 1991;12(1):35-44. doi: 10.1159/000217686.
A lectin-linked immunoradiometric assay (L-IRMA) using 7 different lectins and a monoclonal antibody (MoAb) directed against the protein moiety-specific epitope was developed to detect carcinoembryonic antigen (CEA). The method used for L-IRMA was as follows: certain CEAs that reacted with lectin agarose beads were allowed to bind further to an 125I-labeled anti-CEA MoAb, and the resulting trapped 125I-MoAb was counted in a gamma counter. From the results, CEAs which interacted with Phaseolus vulgaris erythroagglutinin and leukoagglutinin were presumed to contain the complex type of sugar chains. Furthermore, CEAs interacting specifically with wheat germ agglutinin lectin were found in the tested samples, suggesting that the CEA had a hybrid or a complex type of sugar chain as the core structure of the sugar chain, except for that in seminal plasma. These results obtained by L-IRMA were in good accord with the data obtained from serial lectin affinity chromatography. L-IRMA may therefore be a simple method to study the glycoprotein heterogeneities in tumors and in normal subjects.
开发了一种使用7种不同凝集素和针对蛋白质部分特异性表位的单克隆抗体（MoAb）的凝集素连接免疫放射分析（L-IRMA）来检测癌胚抗原（CEA）。L-IRMA所使用的方法如下：使某些与凝集素琼脂糖珠反应的CEA进一步与125I标记的抗CEA MoAb结合，然后在γ计数器中对所得捕获的125I-MoAb进行计数。从结果来看，与菜豆红细胞凝集素和白细胞凝集素相互作用的CEA被推测含有复合型糖链。此外，在测试样品中发现了与麦胚凝集素特异性相互作用的CEA，这表明除精浆中的CEA外，CEA具有杂合型或复合型糖链作为糖链的核心结构。通过L-IRMA获得的这些结果与从系列凝集素亲和色谱法获得的数据高度一致。因此，L-IRMA可能是一种研究肿瘤和正常受试者中糖蛋白异质性的简单方法。
