Sollier E, Cubizolles M, Faivre M, Fouillet Y, Achard J L
Department of Technology for Biology and Health, CEA-LETI-Minatec, 38054 Grenoble, France.
Annu Int Conf IEEE Eng Med Biol Soc. 2009;2009:7030-3. doi: 10.1109/IEMBS.2009.5333314.
Promising microfluidic devices are proposed herein to continuously and passively extract plasma from whole human blood. These designs are based on the red cells lateral migration and the resulting cell-free layer locally expanded by geometric singularities, such as an abrupt enlargement of the channel or a cavity adjacent to the channel. After an explanation of flow patterns, devices are experimentally and biologically validated for plasma extraction. They are also successively optimized with extraction yields up to 17.8% for a 1:20 blood injected at 100 microL/min.
本文提出了一种有前景的微流控装置，用于从全血中连续被动地提取血浆。这些设计基于红细胞的侧向迁移以及由此产生的无细胞层通过几何奇点（如通道的突然扩大或与通道相邻的腔）局部扩展。在解释了流动模式之后，对用于血浆提取的装置进行了实验和生物学验证。它们还经过了连续优化，对于以100微升/分钟注入的1:20血液，提取率高达17.8%。
