使用快速扫描循环伏安法评估丘脑底核刺激引发的纹状体多巴胺释放。

Using fast-scan cyclic voltammetry to evaluate striatal dopamine release elicited by subthalamic nucleus stimulation.

作者信息

Covey Dan P, Garris Paul A

机构信息

School of Biological Sciences and Department of Chemistry, Illinois State University, Normal, IL, USA.

出版信息

Annu Int Conf IEEE Eng Med Biol Soc. 2009;2009:3306-9. doi: 10.1109/IEMBS.2009.5333768.

DOI:10.1109/IEMBS.2009.5333768

PMID:19964299

Abstract

Deep brain stimulation (DBS), an effective neurosurgical therapy for Parkinson's disease (PD), may act via eliciting neurotransmitter release. However, the precise relationships between DBS and neurotransmitter release are not established. One issue in these studies may be analytical limitations of microdialysis and positron emission tomography, the primary measurement technologies employed. Limitations may be overcome by microsensors, which exhibit improved temporal and spatial resolution. Here we assess fast-scan cyclic voltammetry (FSCV) at a carbon-fiber microelectrode (CFM) for monitoring a putative DBS neurotransmitter, dopamine, during stimulation of the subthalamic nucleus (STN), a DBS target for PD. These results, obtained in the anesthetized rat, suggest that real-time microsensors are a suitable approach for testing the neurotransmitter release hypothesis of DBS action.

摘要

深部脑刺激（DBS）是治疗帕金森病（PD）的一种有效神经外科疗法，可能通过引发神经递质释放起作用。然而，DBS与神经递质释放之间的确切关系尚未明确。这些研究中的一个问题可能是所采用的主要测量技术——微透析和正电子发射断层扫描的分析局限性。微传感器可以克服这些局限性，其具有更高的时间和空间分辨率。在此，我们评估了在碳纤维微电极（CFM）上进行的快速扫描循环伏安法（FSCV），以监测在刺激丘脑底核（STN）（PD的一个DBS靶点）期间一种假定的DBS神经递质——多巴胺。在麻醉大鼠身上获得的这些结果表明，实时微传感器是检验DBS作用的神经递质释放假说的一种合适方法。