Zhang Jiayi, Laiwalla Farah, Kim Jennifer A, Urabe Hayato, Van Wagenen Rick, Song Yoon-Kyu, Connors Barry W, Nurmikko Arto V
Department of Physics, Brown University, Providence, RI 02912, USA.
Annu Int Conf IEEE Eng Med Biol Soc. 2009;2009:2046-9. doi: 10.1109/IEMBS.2009.5333947.
Targeted neural excitation coupled with simultaneous multineuron recording is desirable both for studying the real-time dynamics of neural circuits and for prospective clinical treatment of neurological diseases. Optical stimulation of genetically targeted neurons expressing the light sensitive channel protein Channelrhodopsin (ChR2) has recently been reported as a means for millisecond temporal control of neuronal spiking activity with cell-type selectivity. This offers the prospect of enabling local (cellular level) stimulation and the concomitant monitoring of neural activity by extracellular electrophysiological methods, both in the vicinity of and distant to the stimulation site. We report here a novel dual-modality hybrid device which consists of a tapered coaxial optical waveguide ("optrode") directly integrated into a 100 element intra-cortical multi-electrode recording array. The dual-modality array device was used in ChR2 transfected mouse brain slices. Specifically, epileptiform events were reliably optically triggered by the optrode and their spatiotemporal patterns were simultaneously recorded by the multi-electrode array.
靶向神经激发与同步多神经元记录相结合，对于研究神经回路的实时动态以及神经疾病的前瞻性临床治疗都很有必要。最近有报道称，对表达光敏感通道蛋白嗜盐菌视紫红质（ChR2）的基因靶向神经元进行光刺激，是一种对神经元放电活动进行毫秒级时间控制且具有细胞类型选择性的方法。这为通过细胞外电生理方法在刺激部位附近和远处进行局部（细胞水平）刺激以及同时监测神经活动提供了可能。我们在此报告一种新型双模态混合装置，它由直接集成到100元件皮质内多电极记录阵列中的锥形同轴光波导（“光电极”）组成。该双模态阵列装置用于ChR2转染的小鼠脑切片。具体而言，癫痫样事件由光电极可靠地光学触发，其时空模式由多电极阵列同时记录。
