Institute of Bioelectronics (PGI-8/ICS-8) and JARA-Fundamentals of Future Information Technology, Forschungszentrum Jülich, 52425 Jülich, Germany.
J Biomed Opt. 2013 Nov;18(11):111402. doi: 10.1117/1.JBO.18.11.111402.
We present an optoelectrical device capable of in vitro optical stimulation and electrophysiological recording. The device consists of an array of micropixellated InGaN light-emitting diodes coupled to a custom-made ultrathin planar microelectrode array. Cells can be cultured directly on the chip for short- and long-term electrophysiological experiments. To show the functionality of the device, we transfected a cardiomyocyte-like cell line (HL-1) with a light-sensitive protein channelrhodopsin. We monitored action potentials of individual, spontaneously beating, HL-1 cells growing on the chip by extracellular electrical recordings. On-chip optical stimulation was demonstrated by triggering network activity in a confluent HL-1 cell culture and visualized by calcium imaging. We see the potential of our system for electrophysiological experiments with optogenetically modified cells. Optical stimulation can be performed directly on the chip without additional optical components or external light sources.
我们展示了一种光电设备，能够进行体外光学刺激和电生理记录。该设备由一个与定制超薄平面微电极阵列耦合的微像素化 InGaN 发光二极管阵列组成。细胞可以直接在芯片上培养，用于进行短期和长期电生理实验。为了展示该设备的功能，我们用光敏蛋白通道视紫红质转染了一种类似于心肌细胞的细胞系（HL-1）。我们通过细胞外电记录监测在芯片上生长的单个、自发跳动的 HL-1 细胞的动作电位。通过在 HL-1 细胞培养物中触发网络活动，展示了片上光学刺激，并通过钙成像进行可视化。我们看到了我们的系统在光电修饰细胞的电生理实验中的潜力。光学刺激可以直接在芯片上进行，无需额外的光学元件或外部光源。
