Yus Eva, Maier Tobias, Michalodimitrakis Konstantinos, van Noort Vera, Yamada Takuji, Chen Wei-Hua, Wodke Judith A H, Güell Marc, Martínez Sira, Bourgeois Ronan, Kühner Sebastian, Raineri Emanuele, Letunic Ivica, Kalinina Olga V, Rode Michaela, Herrmann Richard, Gutiérrez-Gallego Ricardo, Russell Robert B, Gavin Anne-Claude, Bork Peer, Serrano Luis
Centre for Genomic Regulation (CRG) and Universitat Pompeu Fabra, Avenida Dr. Aiguader 88, 08003 Barcelona, Spain.
Science. 2009 Nov 27;326(5957):1263-8. doi: 10.1126/science.1177263.
To understand basic principles of bacterial metabolism organization and regulation, but also the impact of genome size, we systematically studied one of the smallest bacteria, Mycoplasma pneumoniae. A manually curated metabolic network of 189 reactions catalyzed by 129 enzymes allowed the design of a defined, minimal medium with 19 essential nutrients. More than 1300 growth curves were recorded in the presence of various nutrient concentrations. Measurements of biomass indicators, metabolites, and 13C-glucose experiments provided information on directionality, fluxes, and energetics; integration with transcription profiling enabled the global analysis of metabolic regulation. Compared with more complex bacteria, the M. pneumoniae metabolic network has a more linear topology and contains a higher fraction of multifunctional enzymes; general features such as metabolite concentrations, cellular energetics, adaptability, and global gene expression responses are similar, however.
为了理解细菌代谢组织和调控的基本原理，以及基因组大小的影响，我们系统地研究了最小的细菌之一——肺炎支原体。由129种酶催化的189个反应的人工编辑代谢网络，使得能够设计出一种含有19种必需营养素的确定的、基本培养基。在不同营养浓度下记录了1300多条生长曲线。生物量指标、代谢物测量以及13C-葡萄糖实验提供了关于方向性、通量和能量学的信息；与转录谱分析相结合，实现了对代谢调控的全局分析。然而，与更复杂的细菌相比，肺炎支原体代谢网络具有更线性的拓扑结构，并且含有更高比例的多功能酶；代谢物浓度、细胞能量学、适应性和全局基因表达反应等一般特征是相似的。
