Molecular and Cancer Biology Program, Biomedicum Helsinki, Helsinki, Finland.
Blood. 2010 Jan 28;115(4):906-9. doi: 10.1182/blood-2009-03-212274. Epub 2009 Nov 30.
The lymphatic vasculature is important for the regulation of tissue fluid homeostasis, immune response, and lipid absorption, and the development of in vitro models should allow for a better understanding of the mechanisms regulating lymphatic vascular growth, repair, and function. Here we report isolation and characterization of lymphatic endothelial cells from human intestine and show that intestinal lymphatic endothelial cells have a related but distinct gene expression profile from human dermal lymphatic endothelial cells. Furthermore, we identify liprin beta1, a member of the family of LAR transmembrane tyrosine phosphatase-interacting proteins, as highly expressed in intestinal lymphatic endothelial cells in vitro and lymphatic vasculature in vivo, and show that it plays an important role in the maintenance of lymphatic vessel integrity in Xenopus tadpoles.
淋巴血管系统对于组织液稳态的调节、免疫反应和脂质吸收都很重要，体外模型的发展应该有助于更好地理解调节淋巴血管生长、修复和功能的机制。在这里，我们报告了从人肠中分离和鉴定淋巴管内皮细胞，并表明肠淋巴管内皮细胞与人类皮肤淋巴管内皮细胞具有相关但不同的基因表达谱。此外，我们还鉴定出 liprin beta1，一种 LAR 跨膜酪氨酸磷酸酶相互作用蛋白家族的成员，在体外培养的肠淋巴管内皮细胞和体内的淋巴管中高度表达，并表明它在维持非洲爪蟾幼体的淋巴管完整性中发挥重要作用。
