The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Xuzhou Normal University, Xuzhou, Jiangsu, 221116, PR China.
The Key Laboratory for Microbial Resources of the Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, PR China.
Int J Syst Evol Microbiol. 2010 Nov;60(Pt 11):2524-2528. doi: 10.1099/ijs.0.020099-0. Epub 2009 Dec 4.
A novel strain, designated YIM 61452(T), was isolated from the stem of Maytenus austroyunnanensis collected from the tropical rainforest of Xishuangbanna, Yunnan Province, south-west China. The strain was characterized taxonomically by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 61452(T) belonged to the genus Pseudonocardia and was most closely related to Pseudonocardia alni IMSNU 20049(T) (99.5 %), Pseudonocardia antarctica DVS 5a1(T) (99.5 %) and Pseudonocardia carboxydivorans Y8(T) (99.4 %). The chemotaxonomic properties of strain YIM 61452(T), for example MK-8(H(4)) as the predominant quinone system and iso-C(16 : 0) and iso-C(16 : 1) H as the major fatty acids, were also consistent with its classification within the genus Pseudonocardia. Strain YIM 61452(T) could be differentiated from closely related species of the genus Pseudonocardia by phenotypic and genotypic analysis. DNA-DNA hybridization experiments between strain YIM 61452(T) and closely related reference strains further confirmed that strain YIM 61452(T) represented a novel taxon of the genus Pseudonocardia. Therefore, strain YIM 61452(T) represents a novel species, for which the name Pseudonocardia tropica sp. nov. is proposed. The type strain is YIM 61452(T) (=DSM 45199(T)=CCTCC AA 208018(T)).
一株新的菌株，命名为 YIM 61452(T)，从中国西南云南省西双版纳热带雨林采集的五月茶茎中分离得到。该菌株通过多相分类法进行了分类学特征描述。基于 16S rRNA 基因序列的系统发育分析表明，菌株 YIM 61452(T)属于假诺卡氏菌属，与假诺卡氏菌 Alni IMSNU 20049(T)（99.5%）、假诺卡氏菌南极 DVS 5a1(T)（99.5%）和假诺卡氏菌 carboxydivorans Y8(T)（99.4%）最为密切相关。菌株 YIM 61452(T)的化学分类特性，例如 MK-8(H(4))作为主要醌系统和 iso-C(16 : 0)和 iso-C(16 : 1) H 作为主要脂肪酸，也与其在假诺卡氏菌属中的分类一致。通过表型和基因型分析，菌株 YIM 61452(T)可以与假诺卡氏菌属的密切相关种区分开来。菌株 YIM 61452(T)与密切相关的参考菌株之间的 DNA-DNA 杂交实验进一步证实，菌株 YIM 61452(T)代表了假诺卡氏菌属的一个新分类群。因此，菌株 YIM 61452(T)代表了一个新的物种，建议将其命名为热带假诺卡氏菌。模式菌株为 YIM 61452(T)（=DSM 45199(T)=CCTCC AA 208018(T)）。
