Qin Sheng, Li Jie, Zhao Guo-Zhen, Chen Hua-Hong, Xu Li-Hua, Li Wen-Jun
The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Syst Appl Microbiol. 2008 Oct;31(5):352-7. doi: 10.1016/j.syapm.2008.08.001.
A Gram-positive, aerobic, non-motile, non-acid-alcohol-fast strain, designated YIM 61095(T), was isolated from the root of Maytenus austroyunnanensis collected from a tropical rainforest of Xishuangbanna in Yunnan Province, south-west China. Strain YIM 61095(T) exhibited chemotaxonomic and morphological characteristics that were consistent with members of the genus Saccharopolyspora. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 61095(T) was a member of the genus Saccharopolyspora and was most closely related to Saccharopolyspora flava AS 4.1520(T) (97.7% sequence similarity). The major fatty acids were iso-C(15:0), iso-C(16:0), iso-C(17:0) and anteiso-C(17:0). The predominant quinone detected was MK-9(H(4)). The DNA G+C content was 66.2 mol%. The phenotypic characteristics and DNA-DNA hybridization relatedness data indicated that strain YIM 61095(T) should be distinguished from Saccharopolyspora flava AS 4.1520(T). On the basis of the evidence presented in this study, strain YIM 61095(T) represents a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora endophytica sp. nov. is proposed. The type strain is YIM 61095(T) (=KCTC 19397(T)=CCTCC AA 208003(T)).
从中国西南部云南省西双版纳热带雨林采集的滇南美登木根部分离出一株革兰氏阳性、需氧、不运动、不抗酸酒精的菌株，命名为YIM 61095(T)。菌株YIM 61095(T)表现出与糖多孢菌属成员一致的化学分类和形态特征。基于16S rRNA基因序列的系统发育分析表明，菌株YIM 61095(T)是糖多孢菌属的成员，与黄糖多孢菌AS 4.1520(T)关系最为密切（序列相似性为97.7%）。主要脂肪酸为异-C(15:0)、异-C(16:0)、异-C(17:0)和anteiso-C(17:0)。检测到的主要醌为MK-9(H(4))。DNA G+C含量为66.2 mol%。表型特征和DNA-DNA杂交相关性数据表明，菌株YIM 61095(T)应与黄糖多孢菌AS 4.1520(T)区分开来。基于本研究提供的证据，菌株YIM 61095(T)代表糖多孢菌属的一个新物种，为此提出新名称内生糖多孢菌（Saccharopolyspora endophytica sp. nov.）。模式菌株为YIM 61095(T)（=KCTC 19397(T)=CCTCC AA 208003(T)）。
