Department of Orthodontics, Stomatological College, Fourth Military Medical University, Xi'an 710032, People's Republic of China.
Biomed Mater. 2009 Dec;4(6):065010. doi: 10.1088/1748-6041/4/6/065010.
The aim of this study was to investigate the effects of BMP-2 and dexamethasone (Dex) on osteogenic differentiation of rat dental follicle progenitor cells (RDFCs) seeded on three-dimensional beta-TCP. The alkaline phosphatase (ALP), the calcium and phosphonium, the osteocalcin in media of the third passage RDFCs on biomaterial beta-TCP after 1-3, 3-7, 7-14 days of culture were examined respectively. The growth of cells on the scaffolds was observed by scanning electron microscope (SEM) after 3, 7 days of culture and by implanting in the backs of severe combined immunodeficient (SCID) mice for bone regeneration. The third passage RDFCs could be seen adhered, extended and proliferated on the beta-TCP by scanning electron microscopy. The ALP activity, the calcium and phosphoniums and the osteocalcin content of dexamethasone (10(-8) M) or/and BMP-2 (100 ng ml(-1)) were significantly higher than their existence in the control group. They were the significantly highest among four groups after joint application of BMP-2 and dexamethasone. After 8 weeks of implantation, the percentage of the new bones formed area in the RDFCs+beta-TCP+BMP-2+Dex group was significantly higher than that in the RDFCs+beta-TCP+BMP-2 group. In contrast, beta-TCP, RDFCs+beta-TCP+Dex and control constructs lacked new bone formation by histological staining and histomorphometric analysis. The BMP-2+Dex could significantly promote osteogenic differentiation of RDFCs on beta-TCP. beta-TCP supported fast cellular adhesion, proliferation and differentiation of RDFCs. The feasibility of its application in periodontal tissue engineering was also proved.
本研究旨在探讨骨形态发生蛋白-2（BMP-2）和地塞米松（Dex）对三维β-TCP 种植的大鼠牙周膜祖细胞（RDFCs）成骨分化的影响。分别检测第 3 代 RDFCs 在生物材料β-TCP 上培养 1-3、3-7、7-14 天后培养基中的碱性磷酸酶（ALP）、钙磷、骨钙素。培养 3、7 天后，通过扫描电镜（SEM）观察细胞在支架上的生长情况，并将其植入严重联合免疫缺陷（SCID）小鼠背部进行骨再生。扫描电镜观察到第 3 代 RDFCs 可在β-TCP 上黏附、伸展和增殖。地塞米松（10(-8) M）或/和 BMP-2（100ng/ml(-1)）的 ALP 活性、钙磷和骨钙素含量明显高于对照组。BMP-2 和地塞米松联合应用时，四组中最高。植入 8 周后，RDFCs+β-TCP+BMP-2+Dex 组形成的新骨面积百分比明显高于 RDFCs+β-TCP+BMP-2 组。相反，β-TCP、RDFCs+β-TCP+Dex 和对照构建体缺乏组织学染色和组织形态计量学分析的新骨形成。BMP-2+Dex 可显著促进 RDFCs 在β-TCP 上的成骨分化。β-TCP 支持 RDFCs 的快速细胞黏附、增殖和分化。还证明了其在牙周组织工程中的应用可行性。
