Vichasilp Chaluntorn, Nakagawa Kiyotaka, Sookwong Phumon, Suzuki Yasuyo, Kimura Fumiko, Higuchi Ohki, Miyazawa Teruo
Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Biosci Biotechnol Biochem. 2009 Dec;73(12):2684-9. doi: 10.1271/bbb.90543. Epub 2009 Dec 7.
Mulberry 1-deoxynojirimycin (DNJ, a potent alpha-glycosidase inhibitor) has therapeutic potency against diabetes mellitus. However, the amount of DNJ in mulberry leaves is low (about 0.1%), and a more effective extraction method is needed. Ultrasound-assisted extraction (UAE) was applied in this study for mulberry DNJ extraction, and five factors, the percentage of ethanol in the extraction solvent (x(1)), ratio of the extraction solvent to mulberry sample (x(2)), ultrasonic power (x(3)), extraction temperature (x(4)) and extraction time (x(5)), were investigated by fractional factorial 2((5-1)) design (FFD) to obtain the optimum extraction efficiency (DNJ yield, Y(1)) and extraction productivity (total yield, Y(2)). The results showed that x(2), x(3) and x(5) had significant impact on Y(1) and Y(2), and were further optimized by response surface methodology (RSM). Under the optimized conditions (x(2), x(3) and x(5) of 7 ml/g, 180 W and 260 s, respectively), DNJ-enriched powder (0.8%) was produced with high extraction efficiency (98%) and productivity (20%), enabling this product to be used for nutraceutical purposes.
桑椹1-脱氧野尻霉素（DNJ，一种有效的α-糖苷酶抑制剂）对糖尿病具有治疗效果。然而，桑叶中DNJ的含量较低（约0.1%），因此需要一种更有效的提取方法。本研究采用超声辅助提取（UAE）法提取桑椹中的DNJ，并通过分数析因2((5-1))设计（FFD）研究了提取溶剂中乙醇的百分比（x(1)）、提取溶剂与桑椹样品的比例（x(2)）、超声功率（x(3)）、提取温度（x(4)）和提取时间（x(5)）这五个因素，以获得最佳提取效率（DNJ产率，Y(1)）和提取生产率（总产率，Y(2)）。结果表明，x(2)、x(3)和x(5)对Y(1)和Y(2)有显著影响，并通过响应面法（RSM）进一步优化。在优化条件下（x(2)、x(3)和x(5)分别为7 ml/g、180 W和260 s），制备出了富含DNJ的粉末（0.8%），提取效率高（98%）且生产率高（20%），使得该产品可用于营养保健目的。
