Kimura Toshiyuki, Nakagawa Kiyotaka, Saito Yuko, Yamagishi Kenji, Suzuki Masahiro, Yamaki Kohji, Shinmoto Hiroshi, Miyazawa Teruo
National Agricultural Research Center for Tohoku Region, Fukushima 960-2156, Japan.
J Agric Food Chem. 2004 Mar 24;52(6):1415-8. doi: 10.1021/jf0306901.
A simple and rapid method for determining 1-deoxynojirimycin (DNJ), a potent glucosidase inihibitor present in mulberry leaves (Morus alba and Morus bombysis), by high-performance liquid chromatography coupled to an evaporative light scattering detector (ELSD) has been developed. DNJ was separated from an extract of mulberry leaves on a TSKgel Amide-80 column, which is a representative column for hydrophilic interaction chromatography. During postcolumn detection, DNJ was detected by ELSD and concurrently identified by mass spectrometry. The detection limit was 100 ng. This method is sufficiently sensitive for determining DNJ in mulberry leaves and other related products.
已开发出一种简单快速的方法，通过高效液相色谱-蒸发光散射检测器（ELSD）测定桑叶（白桑和鲁桑）中存在的强效葡萄糖苷酶抑制剂1-脱氧野尻霉素（DNJ）。在TSKgel Amide-80柱（一种用于亲水相互作用色谱的代表性柱）上，从桑叶提取物中分离出DNJ。在柱后检测过程中，通过ELSD检测DNJ，并同时通过质谱进行鉴定。检测限为100 ng。该方法对于测定桑叶及其他相关产品中的DNJ具有足够的灵敏度。
