Nuengchamnong Nitra, Ingkaninan Kornkanok, Kaewruang Wiroje, Wongareonwanakij Sathaporn, Hongthongdaeng Bhinai
Regional Medical Sciences Center Phitsanulok, Department of Medical Sciences, Ministry of Public Health, Phitsanulok 65000, Thailand.
J Pharm Biomed Anal. 2007 Aug 15;44(4):853-8. doi: 10.1016/j.jpba.2007.03.031. Epub 2007 Apr 1.
A novel HPLC-MS/MS method was developed for the quantitative determination of 1-deoxynojirimycin (DNJ), a potent glucosidase inhibitor present in mulberry leaves (Morus alba L.). DNJ was isolated from the mulberry leave extract on a TSKgel Amide-80 column using a mixture of 0.1% formic acid and acetonitrile as a mobile phase at a flow rate of 0.6 ml/min. A triple quadrupole mass spectrometry using electrospray ionization source in a positive ion mode under multiple reaction monitoring with the [M+H]+ ions, m/z 164.4/109.9 was used. The detection limit (S/N=3) was 75 pg and quantitation limit (S/N=10) was 100 pg. The comparison of mulberry leaves of different ages showed that the DNJ level was higher in mulberry shoots than young and mature leaves.
开发了一种新型高效液相色谱-串联质谱法（HPLC-MS/MS），用于定量测定1-脱氧野尻霉素（DNJ），它是桑叶（桑属植物）中一种有效的糖苷酶抑制剂。使用0.1%甲酸和乙腈的混合物作为流动相，流速为0.6 ml/min，在TSKgel Amide-80柱上从桑叶提取物中分离出DNJ。采用电喷雾电离源的三重四极杆质谱仪，在正离子模式下进行多反应监测，监测的是质荷比为164.4/109.9的[M+H]+离子。检测限（S/N=3）为75 pg，定量限（S/N=10）为100 pg。不同年龄桑叶的比较表明，桑树枝条中的DNJ含量高于嫩叶和成熟叶。
