Department of Chemistry, The Pennsylvania State University, University Park, PA, USA.
Lab Chip. 2009 Oct 7;9(19):2796-802. doi: 10.1039/b907942d. Epub 2009 Jul 3.
A microfluidic device was fabricated and characterized for studying cell lysis of Arcella vulgaris, a nonpathogenic amoeba, over time. The device contains a series of chambers which capture cells allowing them to be subsequently exposed to a constant flow of biocidal agent. With this microfluidic system, individual cells are observed as they undergo lysis. This allows high-throughput measurements of individual lysis events, which are not possible with conventional techniques. Differences in lysis and decay times for Arcella were seen at different flow rates and concentrations of benzalkonium chloride, a biocidal detergent. The efficacy of benzalkonium chloride, chlorhexidine digluconate, phenol, sodium dodecyl sulfate, and Triton X-100 were compared, revealing information on their mechanisms of action. The presented device allows cell capture, controlled exposure to chemical biocides, and observation of lysis with single-cell resolution. Observations at the single cell level give insight into the mechanistic details of the lysis of individual Arcella cells vs. the population; decay times for individual Arcella cells were much shorter when compared to a population of 15 cells.
一种微流控设备被制造出来并进行了特性描述，用于研究非致病性变形虫（Arcella vulgaris）的细胞裂解随时间的变化。该设备包含一系列腔室，可以捕获细胞，使它们随后暴露于恒定的杀菌剂流中。通过这种微流控系统，可以观察到单个细胞的裂解过程。这使得可以对单个裂解事件进行高通量测量，而这是传统技术无法实现的。在不同的流速和苯扎氯铵（一种杀菌清洁剂）浓度下，观察到了 Arcella 的裂解和衰减时间的差异。比较了苯扎氯铵、洗必泰葡萄糖酸、苯酚、十二烷基硫酸钠和 Triton X-100 的效果，揭示了它们作用机制的信息。所提出的设备允许细胞捕获、化学杀菌剂的受控暴露以及用单细胞分辨率观察裂解。在单细胞水平的观察提供了对单个 Arcella 细胞与群体裂解的机制细节的深入了解；与 15 个细胞的群体相比，单个 Arcella 细胞的衰减时间要短得多。
