巢式聚合酶链反应在肺外结核诊断中的临床应用。
Clinical usefulness of the nested polymerase chain reaction in the diagnosis of extrapulmonary tuberculosis.
机构信息
Unidad de Investigación Médica en Inmunología e Infectología, Hospital de Infectología, Centro Médico Nacional La Raza, Instituto Mexicano del Seguro Social, 02990 Mexico City, Mexico.
出版信息
Salud Publica Mex. 2009 May-Jun;51(3):240-5. doi: 10.1590/s0036-36342009000300015.
OBJECTIVE
To evaluate the effectiveness of nested polymerase chain reaction (PCR) for diagnosis of extrapulmonary tuberculosis (ETB), as well as the impact of PCR results on clinical management.
MATERIALS AND METHODS
We conducted a study of nested PCR tests in 45 patients and a review of patient hospital files, calculating sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV).
RESULTS
PCR was positive in 51% of cases; PCR sensitivity for diagnosing TB was 86%, specificity was 79%, PPV was 76%, and NPV was 88%. When solely analyzing urine samples, sensitivity and NPV increased to 100%. PCR exerted an influence on management in 27% of patients.
CONCLUSIONS
PCR for rapid diagnosis of extrapulmonary TB has an adequate effect, which improves when performed on urine. The results of PCR exerted an acceptable impact on the clinical management of these patients.
目的
评估巢式聚合酶链反应（PCR）在诊断肺外结核（ETB）中的有效性，以及 PCR 结果对临床管理的影响。
材料与方法
我们对 45 例患者进行了巢式 PCR 检测，并对患者的病历进行了回顾性分析，计算了灵敏度、特异性、阳性预测值（PPV）和阴性预测值（NPV）。
结果
PCR 阳性率为 51%；PCR 诊断结核病的灵敏度为 86%，特异性为 79%，PPV 为 76%，NPV 为 88%。仅分析尿液样本时，灵敏度和 NPV 增加到 100%。PCR 对 27%的患者的治疗管理产生了影响。
结论
PCR 快速诊断肺外结核的效果良好，在检测尿液时效果更佳。PCR 结果对这些患者的临床管理产生了可接受的影响。
Zotero 插件