Sher Institute for Reproductive Medicine, Las Vegas, USA.
J Assist Reprod Genet. 2009 Nov-Dec;26(11-12):629-35. doi: 10.1007/s10815-009-9369-8. Epub 2009 Dec 5.
Cryopreservation of blastocysts, especially those subjected to the trauma due to blastomere biopsy for the purposes of pre-implantation genetic screening (PGS), requires significant optimization. Laboratory and clinical outcomes were compared to determine the effect of two different cryopreservation techniques on the development of human pre-implantation embryos that underwent blastomere biopsy and blastocoel drainage prior to cryopreservation.
Retrospective clinical study.
PATIENT(S): Women who requested cryotransfer of supernumerary blastocysts were analyzed by FISH.
The main outcome measures were post-thaw survival (SR), pregnancy (PR), and implantation (IR). The SR of slowly frozen blastocysts was 83% compared to 97% for vitrified blastocysts. In 160 cases where biopsied embryos were cryotransferred, the results for slowly frozen versus vitrified blastocysts were: SR (71% vs. 95%), PR (23% vs. 37%), and IR (26% vs. 36%, P < 0.05), respectively.
The results revealed that vitrified blastocysts provided higher SR, PR and IR as compared to slowly frozen counterparts.
胚胎的冷冻保存,特别是那些因胚胎活检而受到创伤的胚胎,需要进行大量的优化。本研究比较了两种不同的冷冻保存技术对人胚胎的影响,这些胚胎在冷冻保存前经历了卵裂球活检和囊胚腔抽吸。
回顾性临床研究。
通过 FISH 分析要求冷冻转移多余囊胚的女性。
主要的观察指标是解冻后存活率(SR)、妊娠率(PR)和着床率(IR)。缓慢冷冻的囊胚解冻后存活率为 83%,而玻璃化冷冻的囊胚解冻后存活率为 97%。在 160 例活检胚胎进行冷冻转移的病例中,缓慢冷冻和玻璃化冷冻囊胚的结果分别为:SR(71% vs. 95%)、PR(23% vs. 37%)和 IR(26% vs. 36%),差异有统计学意义(P < 0.05)。
结果表明,与缓慢冷冻相比,玻璃化冷冻的囊胚提供了更高的 SR、PR 和 IR。