[改良贝塞斯达检测法与奈梅亨检测法在检测甲型血友病患者FVII抑制物中的比较]
[Comparison of modified Bethesda assay and Nijmegen assay in detecting FVII inhibitor in patients with hemophilia A].
作者信息
Fan Lian-Kai, Wang Zhi-Wei, Hua Bao-Lai, Su Wei, Wang Shu-Jie, Zhao Yong-Qiang
机构信息
Department of Hematology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
出版信息
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2009 Oct;31(5):551-4.
OBJECTIVE
To compare the sensitivity and practicability of modified Bethesda assay and Nijmegen assay in detecting factor VIII (FVIII) inhibitor.
METHODS
Modified Bethesda assay and Nijmegen assay were used to screen FVIII inhibitors in 237 patients with hemophilia A. The buffer plus universal coagulation reference plasma (UCRP) was used to establish a standard curve for FVIII: C assay in modified Bethesda method, instead of Nijmegen plasma plus FVIII deficiency plasma in Nijmegen method. The cutoff value for positive FVIII inhibitors is > or = 0.6 BU/ml.
RESULTS
The positive rate of FVIII inhibitors was 5.5% (n = 13) when using modified Bethesda assay and was 8.4% (n = 20) when using Nijmegen assay (P > 0.05).
CONCLUSION
Modified standard Bethesda assay is a convenient and feasible method for detecting FVIII inhibitors.
目的
比较改良贝塞斯达法和奈梅亨法检测凝血因子VIII（FVIII）抑制物的敏感性和实用性。
方法
采用改良贝塞斯达法和奈梅亨法对237例甲型血友病患者进行FVIII抑制物筛查。改良贝塞斯达法采用缓冲液加通用凝血参考血浆（UCRP）建立FVIII：C检测标准曲线，而非奈梅亨法中的奈梅亨血浆加FVIII缺乏血浆。FVIII抑制物阳性的临界值为≥0.6 BU/ml。
结果
改良贝塞斯达法检测FVIII抑制物的阳性率为5.5%（n = 13），奈梅亨法为8.4%（n = 20）（P > 0.05）。
结论
改良标准贝塞斯达法是检测FVIII抑制物的一种便捷可行的方法。
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