Vaccine and Infectious Disease Institute, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109-1024, USA.
Expert Rev Anti Infect Ther. 2009 Dec;7(10):1201-21. doi: 10.1586/eri.09.104.
PCR is a very appealing technology for the detection of human pathogens, but the detection of fungal pathogens is particularly challenging. Fungi have cell walls that impede the efficient lysis of organisms and liberation of DNA, which can lead to false-negative PCR results. Conversely, some human pathogens are also ubiquitous environmental saprophytes that can contaminate PCR reagents and cause false-positive results. We examine the quality of PCR-based studies for fungal diagnostics using 42 variables within the Minimum Information for Publication of Quantitative Real-Time PCR Experiments guidelines. This review focuses on taxon-directed PCR assays for the diagnosis of invasive aspergillosis, candidiasis and Pneumocystis pneumonia. Finally, we evaluate broad-range fungal PCR assays capable of detecting a wide spectrum of human pathogens.
PCR 是一种非常有吸引力的技术，可用于检测人类病原体，但检测真菌病原体尤其具有挑战性。真菌具有细胞壁，这会阻碍生物体的有效裂解和 DNA 的释放，从而导致 PCR 结果呈假阴性。相反，一些人类病原体也是无处不在的环境腐生物，可能会污染 PCR 试剂并导致假阳性结果。我们使用定量实时 PCR 实验指南中的 42 个变量来检查基于 PCR 的真菌诊断研究的质量。本综述重点介绍了用于诊断侵袭性曲霉病、念珠菌病和肺孢子菌肺炎的靶向特定分类群的 PCR 检测方法。最后，我们评估了能够检测广泛的人类病原体的广谱真菌 PCR 检测方法。
