Institute of General Zoology and Genetics, Westfalian Wilhelms-University Münster, Münster, Germany.
FEBS Lett. 2010 Feb 5;584(3):493-9. doi: 10.1016/j.febslet.2009.11.096. Epub 2009 Dec 4.
Immune cells navigate through different environments where they experience different mechanical forces. Responses to external forces are determined by the mechanical properties of a cell and they depend to a large extent on the actin-rich cell cortex. We report here that Myo1G, a previously uncharacterised member of class I myosins, is expressed specifically in haematopoietic tissues and cells. It is associated with the plasma membrane. This association is dependent on a conserved PH-domain-like myosin I tail homology motif and the head domain. However, the head domain does not need to be a functional motor. Knockdown of Myo1G in Jurkat cells decreased cell elasticity significantly. We propose that Myo1G regulates cell elasticity by deformations of the actin network at the cell cortex.
免疫细胞在不同的环境中导航，在这些环境中它们会经历不同的机械力。对外力的反应取决于细胞的机械特性，而这些特性在很大程度上取决于富含肌动蛋白的细胞皮质。在这里，我们报告了一个以前未被描述的 I 类肌球蛋白成员 Myo1G ，它在造血组织和细胞中特异性表达。它与质膜相关。这种关联依赖于保守的 PH 结构域样肌球蛋白 I 尾部同源结构域和头部结构域。然而，头部结构域不需要是一个功能性的马达。在 Jurkat 细胞中敲低 Myo1G 会显著降低细胞弹性。我们提出，Myo1G 通过细胞皮质处的肌动蛋白网络的变形来调节细胞弹性。
