Laboratorio de Investigación en Inmunología y Proteómica Hospital Infantil de México, Federico Gómez, Mexico City C.P. 06720, Mexico.
Laboratorio de Producción y Control de Biológicos, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City C.P. 11340, Mexico.
Int J Mol Sci. 2022 Apr 1;23(7):3912. doi: 10.3390/ijms23073912.
Myosin 1g (Myo1g) is a mechanoenzyme associated with actin filaments, expressed exclusively in hematopoietic cells, and involved in various cellular functions, including cell migration, adhesion, and membrane trafficking. Despite the importance of Myo1g in distinct functions, there is currently no monoclonal antibody (mAb) against Myo1g. mAbs are helpful tools for the detection of specific antigens in tumor cells and other tissues. The development of mAbs against targeted dysregulated molecules in cancer cells remains a crucial tool for aiding in the diagnosis and the treatment of patients. Using hybridoma technology, we generated a panel of hybridomas specific for Myo1g. ELISA, immunofluorescence, and Western blot assay results revealed the recognition of Myo1g by these novel monoclonal antibodies in normal and transformed T and B cells. Here, we report the development and application of new monoclonal antibodies against Myo1g for their potential use to detect its overexpression in acute lymphoblastic leukemia (ALL) patients.
肌球蛋白 1g（Myo1g）是一种与肌动蛋白丝相关的机械酶，仅在造血细胞中表达，参与多种细胞功能，包括细胞迁移、黏附和膜运输。尽管 Myo1g 在不同的功能中很重要，但目前还没有针对 Myo1g 的单克隆抗体（mAb）。mAbs 是检测肿瘤细胞和其他组织中特定抗原的有用工具。针对癌细胞中靶向失调分子的 mAbs 的开发仍然是辅助患者诊断和治疗的重要工具。我们使用杂交瘤技术生成了一组针对 Myo1g 的杂交瘤。ELISA、免疫荧光和 Western blot 分析结果显示，这些新型单克隆抗体可识别正常和转化的 T 和 B 细胞中的 Myo1g。在这里，我们报告了针对 Myo1g 的新单克隆抗体的开发和应用，以潜在地用于检测急性淋巴细胞白血病（ALL）患者中其过表达。
