National Water Research Institute, Environment Canada, Burlington, Ontario, Canada L7R 4A6.
J Microbiol Methods. 2010 Feb;80(2):129-33. doi: 10.1016/j.mimet.2009.11.015. Epub 2009 Dec 4.
A DNA microarray with 21 oligonucleotide probes was developed to detect most of the common waterborne protozoan pathogens. The DNA microarray accurately identified 3 test protozoa strains based on the small subunit (SSU) rRNA gene sequence. The detection limit was approximately 1x10(3) target genes, or 50 Cryptosporidium parvum oocysts, per assay. As a parallel study to verify the efficiency of the DNA microarray, a real-time PCR assay was also developed targeting functional genes as well as the SSU rRNA gene. The present protozoa DNA microarray, when combined with our previous bacterial DNA microarray, may serve as a comprehensive water quality monitoring tool.
一种带有 21 个寡核苷酸探针的 DNA 微阵列被开发出来，用于检测大多数常见的水生原生动物病原体。该 DNA 微阵列基于小亚基 (SSU) rRNA 基因序列准确地识别了 3 种测试原生动物菌株。检测限约为每个检测 1x10(3)个靶基因或 50 个微小隐孢子虫卵囊。作为验证 DNA 微阵列效率的平行研究，还开发了一种针对功能基因以及 SSU rRNA 基因的实时 PCR 检测方法。本研究中的原生动物 DNA 微阵列，与我们之前的细菌 DNA 微阵列相结合，可作为一种综合水质监测工具。
