Microbiology Area, DiSTA (Department of Agroenvironmental Sciences and Technologies), Italy.
Fitoterapia. 2010 Jul;81(5):413-9. doi: 10.1016/j.fitote.2009.11.010. Epub 2009 Dec 4.
The present study focused on inhibitory activity of freshly extracted essential oils from three legal (THC<0.2% w/v) hemp varieties (Carmagnola, Fibranova and Futura) on microbial growth. The effect of different sowing times on oil composition and biological activity was also evaluated. Essential oils were distilled and then characterized through the gas chromatography and gas chromatography-mass spectrometry. Thereafter, the oils were compared to standard reagents on a broad range inhibition of microbial growth via minimum inhibitory concentration (MIC) assay. Microbial strains were divided into three groups: i) Gram (+) bacteria, which regard to food-borne pathogens or gastrointestinal bacteria, ii) Gram (-) bacteria and iii) yeasts, both being involved in plant interactions. The results showed that essential oils of industrial hemp can significantly inhibit the microbial growth, to an extent depending on variety and sowing time. It can be concluded that essential oils of industrial hemp, especially those of Futura, may have interesting applications to control spoilage and food-borne pathogens and phytopathogens microorganisms.
本研究关注的是三种合法（THC<0.2%w/v）大麻品种（卡玛格诺拉、菲布兰诺和福图拉）的新鲜提取精油对微生物生长的抑制活性。还评估了不同播种时间对油组成和生物活性的影响。精油经过蒸馏，然后通过气相色谱和气相色谱-质谱进行表征。此后，通过最小抑菌浓度（MIC）测定法，将这些油与标准试剂进行了广谱抑制微生物生长的比较。微生物菌株分为三组：i）革兰氏阳性菌，涉及食源性病原体或胃肠道细菌，ii）革兰氏阴性菌和 iii）酵母，两者都与植物相互作用有关。结果表明，工业大麻的精油可以显著抑制微生物的生长，其抑制程度取决于品种和播种时间。可以得出结论，工业大麻的精油，特别是福图拉的精油，可能在控制腐败和食源性病原体以及植物病原菌微生物方面具有有趣的应用。
