Kanyairita Getrude G, Mortley Desmond G, Collier Willard E, Fagbodun Sheritta, Mweta Jamila M, Uwamahoro Hilarie, Dowell Le'Shaun T, Mukuka Mwamba F
Department of Agriculture and Environmental Sciences, Tuskegee University, Tuskegee, AL 36088, USA.
Department of Chemistry, Tuskegee University, Tuskegee, AL 36088, USA.
Molecules. 2024 Dec 13;29(24):5902. doi: 10.3390/molecules29245902.
Pests and diseases have caused significant problems since the domestication of crops, resulting in economic loss and hunger. To overcome these problems, synthetic pesticides were developed to control pests; however, there are significant detrimental side effects of synthetic pesticides on the environment and human health. There is an urgent need to develop safer and more sustainable pesticides. Industrial hemp is a reservoir of compounds that could potentially replace some synthetic bactericides, fungicides, and insecticides. We determined the efficacy of industrial hemp extracts against pv. (PSTA), pv. (PSTO), and (EC). The study revealed a minimum inhibitory concentration (MIC) of 2.05 mg/mL and a non-inhibitory concentration (NIC) of 1.2 mg/mL for PSTA, an MIC of 5.7 mg/mL and NIC of 0.66 mg/mL for PSTO, and an MIC of 12.04 mg/mL and NIC of 5.4 mg/mL for EC. Time-kill assays indicated the regrowth of at 4 × MIC after 15 h and pv. at 2 × MIC after 20 h; however, pv. had no regrowth. The susceptibility of test bacteria to hemp extract can be ordered from the most susceptible to the least susceptible, as follows: pv. > pv. > . Overall, the data indicate hemp extract is a potential source of sustainable and safe biopesticides against these major plant pathogens.
自从农作物被驯化以来，病虫害就造成了严重问题，导致经济损失和饥饿。为了克服这些问题，人们开发了合成农药来控制害虫；然而，合成农药对环境和人类健康有重大的有害副作用。迫切需要开发更安全、更可持续的农药。工业大麻是一种化合物的储存库，这些化合物有可能取代一些合成杀菌剂、杀真菌剂和杀虫剂。我们测定了工业大麻提取物对马铃薯晚疫病菌致病疫霉（PSTA）、致病疫霉（PSTO）和番茄早疫病菌（EC）的功效。研究表明，PSTA的最低抑菌浓度（MIC）为2.05毫克/毫升，非抑菌浓度（NIC）为1.2毫克/毫升；PSTO的MIC为5.7毫克/毫升，NIC为0.66毫克/毫升；EC的MIC为12.04毫克/毫升，NIC为5.4毫克/毫升。时间杀菌试验表明，15小时后致病疫霉在4倍MIC浓度下重新生长，20小时后致病疫霉在2倍MIC浓度下重新生长；然而，致病疫霉没有重新生长。受试细菌对大麻提取物的敏感性从最敏感到最不敏感依次为：致病疫霉>PSTO>番茄早疫病菌。总体而言，数据表明大麻提取物是针对这些主要植物病原体的可持续且安全的生物农药的潜在来源。
