Faculty of Health and Society, Malmö University, SE-205 06 Malmö, Sweden.
J Colloid Interface Sci. 2010 Apr 1;344(1):44-7. doi: 10.1016/j.jcis.2009.12.042. Epub 2009 Dec 28.
The objective of the investigation was to study how different surface cleaning procedures affect the subsequent adsorption of bovine serum albumin (BSA) on silica. Ellipsometry was used to monitor the adsorbed amount and thickness of the protein films in situ at physiological buffer conditions. Plasma treatment was found to effectively reduce the amount of BSA on silica and on surfaces cleaned with hydrogen peroxide solutions (RCA method) adsorption was essentially absent. On clean silica surfaces the ellipsometric data furthermore suggest that a depletion zone is present at the surface where the BSA concentration is lower than in the bulk solution. Additional experiments showed that calcium did not promote the adsorption of BSA and that the adsorption of human serum albumin (HSA) is essentially absent on clean silica surfaces in agreement with the results obtained for BSA. The presented work contributes to the general understanding of protein adsorption as well as provides information on how to control serum albumin adsorption on silica based biomedical devices.
本研究旨在探讨不同的表面清洁程序如何影响牛血清白蛋白（BSA）在二氧化硅上的后续吸附。采用椭圆光度法在生理缓冲条件下原位监测蛋白质薄膜的吸附量和厚度。等离子体处理可有效减少二氧化硅和经过氧化氢溶液（RCA 法）清洁的表面上的 BSA 量，吸附基本不存在。在清洁的二氧化硅表面上，椭圆光度法数据进一步表明，在表面存在耗尽区，BSA 浓度低于本体溶液。进一步的实验表明，钙不会促进 BSA 的吸附，并且 HSA 在清洁的二氧化硅表面上的吸附基本不存在，这与 BSA 的结果一致。本研究工作有助于更好地理解蛋白质吸附，同时提供了关于如何控制基于二氧化硅的生物医学设备中血清白蛋白吸附的信息。
