Lab. of Biomechanics and Biocalorimetry (LOB(2)), Faculty of Medicine, University of Basel, c/o Biozentrum - Pharmazentrum, Klingelbergstrasse 50-70, 4056 Basel, Switzerland.
Tuberculosis (Edinb). 2010 Jan;90(1):57-9. doi: 10.1016/j.tube.2009.11.001. Epub 2009 Dec 6.
Detection of mycobacterial infection can be achieved by different means; however, culture-based methods remain the gold standard. In this paper, we present a new culture-based method using real-time microcalorimetric detection of growth of Mycobacterium species including Mycobacterium tuberculosis. Microcalorimetric detection of heat production by 6 different growing species of Mycobacterium was achieved between 20 and 310h depending on their type (fast vs. slow-growing mycobacteria) and initial concentration. This study demonstrates that microcalorimetric detection of mycobacterial growth is a potential advantageous alternative to methods using fluorescent or radiolabeled growth indicators.
可以通过不同的方法来检测分枝杆菌感染；然而，基于培养的方法仍然是金标准。在本文中，我们提出了一种新的基于培养的方法，使用实时微量热法检测包括结核分枝杆菌在内的分枝杆菌属的生长。根据其类型（快速生长分枝杆菌与缓慢生长分枝杆菌）和初始浓度，6 种不同生长类型的分枝杆菌的产热情况在 20 至 310 小时之间得到了微量热法的检测。本研究表明，分枝杆菌生长的微量热检测是一种有潜力的替代方法，可以替代使用荧光或放射性标记生长指示剂的方法。
