大鼠肾脏中的皮质素转运蛋白：合成皮质素转运蛋白的多核糖体的亚细胞分布

Transcortin in rat kidney: subcellular distribution of transcortin-synthesizing polyribosomes.

作者信息

Kraujelis K, Ulinskaite A, Meilus V

机构信息

Laboratory for Study of Biologically Active Substances, Academy of Sciences of Lithuania, Mokslininku, U.S.S.R.

出版信息

J Steroid Biochem Mol Biol. 1991 Jan;38(1):43-7. doi: 10.1016/0960-0760(91)90399-p.

DOI:10.1016/0960-0760(91)90399-p

PMID:1997123

Abstract

The [3H]corticosterone-transcortin complexes from kidney cytosol show elution positions on DEAE-cellulose identical to serum transcortin. The incorporation of 14C-labeled amino acids into anti-transcortin-precipitable material of kidney slices has been measured and compared with that of serum transcortin. It was established that kidney synthesized transcortin with an apparent molecular weight of 66 kDa on SDS-electrophoresis which resembles serum corticosteroid-binding globulin. Studies on the binding of [125I]anti-transcortin-IgG to membrane-bound rat kidney polyribosomes revealed an association of [125I]anti-transcortin-IgG with a discrete polyribosome fraction in the heavy polyribosome region; free polyribosomes were devoid of antigenic material able to bind antibodies to transcortin.

摘要

来自肾细胞溶质的[3H]皮质酮-运皮质素复合物在DEAE-纤维素上的洗脱位置与血清运皮质素相同。已对14C标记的氨基酸掺入肾切片抗运皮质素可沉淀物质的情况进行了测定，并与血清运皮质素进行了比较。结果确定，肾脏合成的运皮质素在SDS电泳上的表观分子量为66 kDa，与血清皮质类固醇结合球蛋白相似。对[125I]抗运皮质素-IgG与膜结合的大鼠肾多核糖体的结合研究表明，[125I]抗运皮质素-IgG与重多核糖体区域中的一个离散多核糖体部分相关联；游离多核糖体缺乏能够结合抗运皮质素抗体的抗原物质。