Krauialis K I, Ulinskaĭte A P, Meĭlus V I
Biokhimiia. 1991 Jan;56(1):94-9.
During chromatography of renal tissue cytosolic proteins on DEAE-cellulose the protein specifically binding [3H]corticosterone is eluted within the potassium phosphate concentration range of 0.08-0.10 M. Analysis of kidney slices revealed the synthesis of [3H]transcortin whose electrophoretic mobility was close to that of the blood plasma protein. Using radioimmunochemical methods, it has been found that transcortin-specific [125I]IgG antibodies interact with growing polypeptide chains of membrane-bound polyribosomes. Free polyribosomes do not bind antibodies against transcortin.
在对肾组织胞质蛋白进行DEAE - 纤维素层析时，特异性结合[³H]皮质酮的蛋白在磷酸钾浓度范围为0.08 - 0.10 M时被洗脱。对肾切片的分析显示[³H]皮质素转运蛋白的合成，其电泳迁移率与血浆蛋白相近。使用放射免疫化学方法发现，皮质素转运蛋白特异性的[¹²⁵I]IgG抗体与膜结合多核糖体的正在生长的多肽链相互作用。游离多核糖体不结合抗皮质素转运蛋白的抗体。
